SEMINAL FLUID ANALYSIS
EXAMINATION PARAMETER
MACROSCOPIC EXAMINATION
1) Time of arrival
2) Appearances of the specimen
3) Viscosity/liquefaction
4) Volume of the specimen
MICROSCOPIC EXAMINATION
MOTILITY
a. Quantitative motility
b. Qualitative motility
Sperm count
HOW TO COLLECT SAMPLE
1) The sample should be collected after abstaining from sex for 2-5 days
2) The sample should be collected in the laboratory by well-trained laboratory health personnel via masturbation
3) The sample must be delivered to the laboratory within 2 hours after collection when the sample is collected outside of the laboratory
4) The used of condom is not allowed, but it can be used if he condom if free of spermicides or lubricant
5) The sample should be discarded when leakage where found in the container during transportation of the sample to the laboratory
6) The container must be free and sterile and the name of the patient must be fully written on it.
7) The temperature range should be maintain, the average temperature should be less that 20oC and it should not be more than 40oC for exposure
8) The examination of single specimen should not be encourage, at list two to three specimen should be collected a week or two week interval.
NORMAL SEMEN CHARACTERISTIC
Freshly ejaculated semen should have the following characteristic;
1) Highly viscid opaque and creamy
2) White in colour and have a distinct musky or sickly smell
3) The specimen should liquefy within 20 minute after collection
4) On liquefaction the specimen may change slightly viscous
5) The specimen may be turbid and alkaline with Ph 7.7
6) The total volume is measure, which after 2-5 days abstinence from sex, the volume should be from 2-3ml
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