PARAMETER PROCEDURE
1 TIME OF ARIVAL
2 APEARANCE
3 VISCOSITY/LIQUEFACTION
SEMINAL FLUID ANALYSIS PROCEDURE
A sterile pipette should be used to suck up the fluid and then release it, if the liquid drop slowly one by one, it viscosity is normal. Then report normal viscosity.
But if the fluid fallout from the pipette without dropping, then report watery semen
NB: increase viscosity of the sperm can inhibit the sperm motility.
DOUNLAOD OUR FREE BOOK PROCEDURE FOR WIDAL TEST
4 VOLUME
Liquefy semen can be measure with 10ml or 5ml pipette. For viscosity semen, wide mouth pipette should be used.
NB: Less volume of the spermatozoa may be due to lack of abstinence from sex, ofwhich it can also interfere with the sperm count.
MICROSCOPY EXAMINATIION
MORTILITY
Include Quantitative motility and Qualitative motility
Normal sperm Quantitative motility is from 60% upward
LABORATORY PROCEDURE
One drop of liquefied mixed semen should be place on a clean grease free slide, cover with cover slip and examine microscopically with x10 objective lens with iris diaphragm close, then focus properly with x40 objective.
WHO (world health organization) recommend for motility analysis.
QUANTITATIVE MOTILITY
This should be determined by counting both number of motile and non motile for at list 10 randomly selected fields, and at list 100 must be counted.
The percentage of motile sperm is calculated from the means percentage motility for the entire field counted. This is then adjusted to the nearest 5% (e.g 73% = 75%).
QUALITATIVE MOTILITY
This are determine by the percentage progress movement of the sperm under examination.
The result should be reported as follow
1) NON: when there are no progressive movement seen
2) POOR: when the number of the progressive movement is less than non motile
3) GOOD: when the number of the progressive movement is slightly higher than the number of non motile
4) EXCELLENT: when almost or all the number of sperm are motile
SPERM COUNT
Sperm count is carried out with the use of haemocytometer. Also electronic coulter counter can be used if it is available.
METHOD
The specimen should be diluted in 1:20 by using WBC (white blood cell) pipette.
Draw 0.5 mark of semen dilute it with diluting fluid of 11 mark.
Alternatively; micro pipette can be used, when using micro pipette,
Measure 10µl, 50µl, or 100µ volume can be used to make 1:20 dilution in a test tube.
NB: the diluting fluid should immobilize and preserve sperms.
COMMON DILUTING FLUID COMPOSITION
1. Sodium bicarbonate (NaHCO3) 5g
2. Formalin (neutral) 1ml
3. Distilled water 100ml
NB: this diluting is satisfactory for both bright and phase contrast microscopy.
But 0.5ml of saturated aqueous solution of gentian violet can be added for light microscopy.
Also tap water that is not acidic can be used for dilution.
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